Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJID (6) Genet. Mol. Biol. (2) BJM (1) Ciência Rural (1) Autor Lunge,Vagner Ricardo (10) Simon,Daniel (7) Ikuta,Nilo (5) Wolf,Jonas Michel (3) Alves,Camila Fernanda da Silveira (2) Boeira,Thaís da Rocha (2) Coser,Janaina (2) Fonseca,André Salvador Kazantzi (2) Grott,Camila Schultz (2) Wortmann,André Castagna (2) Bona,Laura Renata de (1) Borchardt,Alessandra Caroline (1) Béria,Jorge Umberto (1) Cecconi,Maria Cristina Piccoli (1) Celmer,Álvaro José (1) Cerbaro,Kamila (1) Cirolini,Adriana Maria (1) Fagundes,Nelson Jurandi Rosa (1) Fonseca,André (1) Fraga,Aline Padilha (1) Mais... Palavra-chave Epidemiology (2) Antibiotic resistance (1) Arcobacter butzleri (1) Bloating (1) Carcaças (1) Cervical cancer (1) Cross-sectional studies (1) DNA probes (1) Dyspepsia (1) Enteritidis (1) Gastrointestinal diseases (1) HBV genotypes (1) HBV infection (1) HIV/HCV co-infected patients (1) HPV persistence (1) HPV polymerase chain reaction polymorphism (1) HPV prevalence (1) IFNL4 genotypes (1) Lactose intolerance (1) Matrizes descartadas (1) Mais... Tipo do documento Info:eu-repo/semantics/article (7) Info:eu-repo/semantics/other (3) Ano 2018 (3) 2016 (2) 2014 (2) 2013 (1) 2011 (1) 2003 (1) Mais... País Brazil (10) Idioma Inglês (9) Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 10 Primeira ... 1 ... Última A Multiplex real-time PCR for detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from Brazilian commercial poultry flocks BJM Fraga,Aline Padilha; Vargas,Tatiana de; Ikuta,Nilo; Fonseca,André Salvador Kazantzi; Celmer,Álvaro José; Marques,Edmundo Kanan; Lunge,Vagner Ricardo. Mycoplasma gallisepticum (MS) and Mycoplasma synoviae (MS) are important avian pathogens and cause economic losses to the poultry industry. Molecular biology techniques are currently used for a rapid detection of these pathogens and the adoption of control measures of the diseases. The aim of this study was to develop and validate a technique for simultaneous detection of MG and MS by multiplex real time polymerase chain reaction (PCR). The complete assay (Multiplex MGMS) was designed with primers and probes specific for each pathogen and developed to be carried out in a single tube reaction. Vaccines, MG and MS isolates and DNA from other Mycoplasma species were used for the development and validation of the method. Further, 78 pooled clinical samples... Tipo: Info:eu-repo/semantics/article Palavras-chave: Real-time PCR; Mycoplasma gallisepticum; Mycoplasma synoviae. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200028 Cervical human papillomavirus infection and persistence: a clinic-based study in the countryside from South Brazil BJID Coser,Janaina; Boeira,Thaís da Rocha; Wolf,Jonas Michel; Cerbaro,Kamila; Simon,Daniel; Lunge,Vagner Ricardo. Abstract Human papillomavirus (HPV) infection is common in sexually active women and viral persistence may cause intraepithelial lesions and eventually progress to cervical cancer (CC). The present study aimed to investigate epidemiological factors related to HPV infection and to evaluate viral persistence and CC precursor lesions frequencies in women from a city in the countryside of South Brazil. Three hundred women were recruited from a primary public health care clinic. The patients were interviewed and underwent sampling with cervical brushes for HPV-DNA detection/typing by a PCR-based assay and cytological analysis by Pap smear test. HPV was detected in 47 (15.7%) women. HPV infection was significantly associated with young age (<30 years) and low... Tipo: Info:eu-repo/semantics/article Palavras-chave: HPV prevalence; HPV persistence; Cervical cancer; Epidemiology. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000100061 HBV epidemiology and genetic diversity in an area of high prevalence of hepatitis B in southern Brazil BJID Paoli,Juliana de; Wortmann,André Castagna; Klein,Mirelli Gabardo; Pereira,Vagner Reinaldo Zingalli Bueno; Cirolini,Adriana Maria; Godoy,Bibiane Armiliato de; Fagundes,Nelson Jurandi Rosa; Wolf,Jonas Michel; Lunge,Vagner Ricardo; Simon,Daniel. ABSTRACT Background Hepatitis B virus (HBV) infection is a major public health problem in Brazil. HBV endemicity is usually moderate to low according to geographic regions, and high prevalence of this virus has been reported in people of some specific Brazilian counties, including those with a strong influence of Italian colonization in southern Brazil. Analysis of HBV diversity and identification of the main risk factors to HBV infection are necessary to understand hepatitis B epidemiology in these high prevalence regions in southern Brazil. Objective To investigate epidemiological characteristics and HBV genotypes and subgenotypes circulating in a specific city with high HBV prevalence. Methods A cross-sectional study was performed with 102 HBV... Tipo: Info:eu-repo/semantics/article Palavras-chave: HBV infection; Epidemiology; HBV genotypes; Risk factor; Transmission; Cross-sectional studies. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400294 Human papillomavirus detection and typing using a nested-PCR-RFLP assay BJID Coser,Janaina; Boeira,Thaís da Rocha; Fonseca,André Salvador Kazantzi; Ikuta,Nilo; Lunge,Vagner Ricardo. BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene. METHODS: Analysis of published DNA sequence of mucosal HPV types to select sequences of new primers. Design of an original nested-PCR assay using the new primers pair selected and classical MY09/11 primers. HPV detection and typing in cervical samples using the nested-PCR-RFLP assay. RESULTS: The nested-PCR-RFLP assay detected and typed HPV in cervical samples. Of the total of 128 clinical samples submitted to simple PCR and nested-PCR for detection of HPV,... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA probes; HPV polymerase chain reaction polymorphism; Restriction fragment length. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500009 Increasing prevalence and dissemination of invasive nontyphoidal Salmonella serotype Typhimurium with multidrug resistance in hospitalized patients from southern Brazil BJID Reis,Rafael Oliveira dos; Souza,Margarida Neves; Cecconi,Maria Cristina Piccoli; Timm,Loeci; Ikuta,Nilo; Simon,Daniel; Wolf,Jonas Michel; Lunge,Vagner Ricardo. ABSTRACT Introduction: Nontyphoidal Salmonella serotypes are the main cause of human food-borne infection, including several hospitalization cases in the developing countries. Aim: To detect the main serotypes and to characterize the antibiotic resistance of human non-enteric and enteric nontyphoidal Salmonella from clinical isolates in Brazil. Methods: Salmonella serotypes were identified by microbiological and molecular methods. Susceptibility testing to antibiotics was performed by agar disk diffusion. Real-time PCRs were carried out for the detection of the genus Salmonella as well as serotypes Typhimurium and Enteritidis. Results: A total of 307 nontyphoidal Salmonella were isolated from 289 different patients in a reference laboratory (LACEN-RS)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Antibiotic resistance; Typhimurium; Enteritidis. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000500424 Interferon lambda 4 (IFNL4) gene polymorphism is associated with spontaneous clearance of HCV in HIV-1 positive patients Genet. Mol. Biol. Alves,Camila Fernanda da Silveira; Grott,Camila Schultz; Lunge,Vagner Ricardo; Béria,Jorge Umberto; Tietzmann,Daniela Cardoso; Stein,Airton Tetelbom; Simon,Daniel. Abstract Approximately one-third of the individuals infected with human immunodeficiency virus type 1 (HIV-1) are co-infected with hepatitis C virus (HCV). Co-infected patients have an increased risk for developing end-stage liver diseases. Variants upstream of the IFNL3 gene have been associated with spontaneous and treatment-induced clearance of HCV infection. Recently, a novel polymorphism was discovered, denoted IFNL4 ΔG > TT (rs368234815), which seems to be a better predictor of spontaneous clearance than the IFNL4 rs12979860 polymorphism. We aimed to determine the prevalence of the IFNL4 ΔG > TT variants and to evaluate the association with spontaneous clearance of HCV infection in Brazilian HIV-1 patients. The IFNL4 ΔG > TT genotypes were... Tipo: Info:eu-repo/semantics/other Palavras-chave: IFNL4 genotypes; HIV/HCV co-infected patients; Spontaneous clearance. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300374 Isolamento de Arcobacter butzleri de músculos de carcaças de suínos de terminação e de matrizes descartadas abatidos em um matadouro no Estado do Rio Grande do Sul, Brasil Ciência Rural Oliveira,Sérgio José de; Ikuta,Nilo; Lunge,Vagner Ricardo; Fonseca,André; Moraes,Hamilton Luiz; Kuchenbecker,Beatris Sontaz; Passos,Daniel Thompson. Foram colhidos fragmentos de músculos da carcaça de 74 suínos de abate e de 150 porcas descartadas de granjas de ciclo completo, abatidos em frigorífico no Rio Grande do Sul. Os materiais foram inoculados em meio EMJH (Ellinghausen, MacCullough, Johnson &amp; Harris), incubados a 30ºC e o cultivo foi passado a placas de agar sangue através de membrana de 0,45µm. Foram obtidas 69 amostras de Arcobacter sp, classificadas por PCR como A. butzleri, respectivamente de suínos de abate (21 isolamentos, 29,3%) e matrizes descartadas (48 isolamentos, 32%). Tipo: Info:eu-repo/semantics/article Palavras-chave: Carcaças; Suínos de abate; Matrizes descartadas; Arcobacter butzleri. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000500015 Low prevalence of human immunodeficiency virus and hepatitis C virus co-infection in a medium size city in southern Brazil BJID Librelotto,Carina Sperotto; Simon,Daniel; Ikuta,Nilo; Lunge,Vagner Ricardo. Tipo: Info:eu-repo/semantics/other Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600689 The association between adult-type hypolactasia and symptoms of functional dyspepsia Genet. Mol. Biol. Wortmann,André Castagna; Simon,Daniel; Mazzoleni,Luiz Edmundo; Sander,Guilherme Becker; Francesconi,Carlos Fernando de Magalhães; Nabinger,Débora Dreher; Grott,Camila Schultz; Rech,Tássia Flores; Mazzoleni,Felipe; Lunge,Vagner Ricardo; Bona,Laura Renata de; Milbradt,Tobias Cancian; Silveira,Themis Reverbel da. Abstract Functional dyspepsia and lactose intolerance (adult-type hypolactasia, ATH) are common conditions that may coexist or even be confounded. Their clinical presentation can be similar, however, lactose intolerance does not form part of the diagnostic investigation of functional dyspepsia. Studies on the association between functional dyspepsia and ATH are scarce. This study aimed to evaluate whether ATH is associated with symptoms of functional dyspepsia. Patients fulfilling the Rome III diagnostic criteria for functional dyspepsia underwent genetic testing for ATH. Dyspeptic symptoms were evaluated and scored according to a validated questionnaire. The diagnostic criteria for ATH was a CC genotype for the -13910C/T polymorphism, located upstream of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bloating; Dyspepsia; Gastrointestinal diseases; Lactose intolerance; Single nucleotide polymorphism. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100092 Viral genotypes and human rs12979860 polymorphism of the IFNL3 gene in hepatitis C infected patients in Southern Brazil BJID Frizon,Kelen; Alves,Camila Fernanda da Silveira; Borchardt,Alessandra Caroline; Lunge,Vagner Ricardo; Simon,Daniel. Tipo: Info:eu-repo/semantics/other Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000200229 Registros recuperados: 10 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco